Cytotoxic and thrombolytic activity of the aerial part of cestrum diurnum l. (solanaceae)

Abstract

The methanol extract of cestrum diurnum l. belonging to the family solanaceae has been investigated for the presence of its secondary metabolites and evaluation of biological activities of the crude extractive for the brine shrimp lethality bioassay and thrombolytic activity. The methanol extract of the plant indicated the presence of alkaloids, steroids and tannin. The plant exhibited significant cytotoxic effect on brine shrimp lethality bioassay, where the plant extracts showed LC50 of 0.074 μg/ml and LC90 of 4.85μg/ml after 2 hrs and later the all shrimp died after 4hrs at the same concentration. For the standard chloramphenicol LC50 and LC90 were found 2.56μg/ml and 4.82μg/ml respectively after 2hrs. After 4hrs LC50 and LC90 were found 1.772μg/ml and 4.364μg/ml respectively. The percentages of clot iyses were found 8.783% for test sample, 2.44% for blank and 80.43% for standard streptokinase in thrombolytic assay.